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This testing requires the adding of a chemical agent to the sample in order to identify which drugs have been consumed, which is a process known as derivatization

Abbreviations COPD: Chronic obstructive pulmonary disease CC10: Club cell 10 kDa secretory protein CS: Cigarette smoking WT: Wild-type CSE: Cigarette smoke extract mIHC: Multiplexed immunohistochemistry ELISA: Enzyme-linked immunosorbent assay BALF: Bronchoalveolar lavage fluid IF: Immunofluorescence SEM: Scanning electron microscopy TEM: Transmission electron microscopy NC: Negative control MAST: Mean alveolar septal thickness WBC: White blood cell References Agust A, Celli BR, Criner GJ, et al